Similar users
Stephen Charles Kowalczykowski
Distinguished Professor of Microbiology & Molecular Genetics, and of Molecular & Cellular Biology
Institution:
Kowalczykowski Lab
Davis, CA, United States
https://www.mysciencework.com/profile/stephen.charles.kowalczykowski
Summary
Published articles Show More
Rad52 protein has a second stimulatory role in DNA strand exchange that complements replication protein-A function.
Published in Journal of Biological Chemistry
Rad52 protein plays a central role in double strand break repair and homologous recombination in Saccharomyces cerevisiae. We have identified a new mechanism by which Rad52 protein stimulates Rad51 protein-promoted DNA strand exchange. This function of Rad52 protein is revealed when subsaturating amounts (relative to the single-stranded DNA concent...
Decatenation of DNA by the S. cerevisiae Sgs1-Top3-Rmi1 and RPA complex: a mechanism for disentangling chromosomes.
Published in Molecular Cell
Genetic evidence indicates that Saccharomyces cerevisiae Sgs1, Top3, and Rmi1 resolve topologically linked intermediates arising from DNA replication and recombination. Using purified proteins, we show that Sgs1, Top3, Rmi1, and replication protein A (RPA) coordinate catenation and decatenation of dsDNA through sequential passage of single strands ...
Direct visualization of RecBCD movement reveals cotranslocation of the RecD motor after chi recognition.
Published in Molecular Cell
In Escherichia coli, chi (5 -GCTGGTGG-3 ) is a recombination hotspot recognized by the RecBCD enzyme. Recognition of chi reduces both nuclease activity and translocation speed of RecBCD and activates RecA-loading ability. RecBCD has two motor subunits, RecB and RecD, which act simultaneously but independently. A longstanding hypothesis to explain t...
Experience
Professor of Microbiology and of Molecular Biology and Cell Biology Since 1991
University of California, Davis (Davis, CA US)
Postdoctoral Fellow - 1981
University of Oregon (Eugene, OR US)
Interactions of Bacteriophage T4-Coded Gene 32 Protein with Nucleic Acids
Education
B.S., Chemistry - 1972
Rensselaer Polytechnic Institute (Troy, NY US)
Substrate Binding Order of Sheep Brain Glutamine Synthetase
Ph. D, Chemistry - 1976
Georgetown University (Washington, DC US)
Physical-Chemical Studies of Sickle Cell Hemoglobin
Contact me
530-752-5938
