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Naveen Kumar Singh

Naveen Kumar Singh

Postdoctoral Research Associate

Discipline: Biochemistry
152 McCourtney Hall, University of Notre Dame, Notre Dame, 46556. United States

Summary

Naveen Kumar Singh received his B.Sc. in Biotechnology (2007) at University of Pune (Pune, India), M.Sc. in Biotechnology (2009) at Jaipur National University (Jaipur, India), and Ph.D. in Biochemical Engineering (2016) at Jacobs University Bremen (Bremen, Germany).


During his Ph.D., Naveen worked under the supervision of Prof. Marcelo Fernández-Lahore. His research dealt with designing experiments for developing fiber-based and cryogel-based adsorbents for capturing large therapeutic biomolecules (proteins, plasmids, and monoclonal antibodies). He had successfully evaluated the in-house fiber- and cryogel-based chromatographic adsorbents with the commercially available adsorbents and the in-house adsorbents had shown similar or higher productivities compared to the commercial adsorbents.


In February 2017, Naveen joined the group of Prof. Merlin L. Bruening in the Department of Chemical and Biomolecular Engineering at the University of Notre Dame as a Postdoctoral Research Associate. His current research focuses on developing novel bioseparation processes by introducing polyelectrolyte multilayer films onto membranes/monoliths for the purification of biotherapeutics like monoclonal antibodies.

Published articles Show More

Experience

Postdoctoral Research Associate Since February 2017

University of Notre Dame (Notre Dame US)

  • Developed porous membranes and monoliths for rapidly capturing monoclonal antibodies.
  • Evaluated mass transfer properties of monoliths- and membrane-based ion-exchangers using model proteins.
  • Successfully developed/evaluated enzymatic deglycosylation of monoclonal antibody using LC-MS.
  • Extensive hands-on skills in middle down/bottom up approach for peptide mapping, protein sequences identification, and protein posttranslational modifications (PTMs) characterization using mass spectrometry.

Research Associate May 2016 - October 2016

Jacobs University Bremen gGmbH (Bremen DE)

  • Tailored the gPore fiber-based system in the recovery and purification of monoclonal antibodies.
  • Scaled a chromatography method (1 mL to 250 mL) for monoclonal antibodies purification.

Research Associate June 2011 - January 2016

Jacobs University Bremen gGmbH (Bremen DE)

  • Developed, modified, and evaluated the performance of gPore fiber-based materials, which can be functionalized and used to purify a range of bioproducts efficiently.
  • Successfully designed a single step downstream process for B-phycoerythrin purification and scaled it from 1 mL to 250 mL chromatographic column.
  • Established lab-scale chromatography methods for recombinant proteins, monoclonal antibodies, pDNA, and mRNA purification.
  • Evaluated mass transfer properties of gPore fiber- and crygoel-based adsorbents using model proteins and interpreted that the mass transfer zones obtained with the gPore fibers and cryogels were neither affected by the linear flow velocity nor the protein concentration in the mobile phase. 
  • Trained 6 undergraduate, master, and Ph.D. students for the conduct of independent research.

Trainee/Executive Trainee July 2010 - January 2011

Wockhardt Ltd (Aurangabad IN)

  • Performed large-scale purification of recombinant insulin glargine under cGMP for clinical trials.
  • Carried out solubilization of inclusion bodies, refolding and final conversion of proinsulin to insulin.
  • Operated different column operations (Ion-, buffer-, cation-exchange chromatography, and RP-HPLC).
  • Collaborated with project manager in shortlisting better trypsin enzyme by running a couple of experiments.
  • Followed and maintained cGMP, standard operating procedures, health, and safety policies.

Education

Contact me

152 McCourtney Hall, University of Notre Dame
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