Doctor of Philosophy (Ph.D.)-Biotechnology
July 2009 - October 2014
My Ph.D. research focused on the mechanisms involved in Vitex Sp. an unexplored medicinal plant on Liver Cancer. In my research, the plant samples were collected and following assays were performed to check the pharmacological significance of Vitex Sp. through phytochemical analysis, Antimicrobial, Antifungal, Minimal inhibitory concentrations, Invitro and invivo Antioxidant, anticancer properties, High-Performance Thin Layer Chromatography (HPTLC), Preparative Thin Layer Chromatography. As an integral part of my research, I worked on the chemopreventive action of Vitex sp. In Sprague Dawley rats and analysis in which I had performed In vivo assays like Toxicity studies (Acute and sub-acute), Inflammatory studies (Carrageen induced, cotton pellet, glass rod methods), Inflammatory bowel disease (IBD), Anticancer activities (DMBA, DAL model), Hepatoprotective (CCL4 induced, Paracetamol induced) and In vitro cell line studies of medicinal plants. I had presented that Vitex Sp. acts as a novel anticancer agent in Hepatic Carcinoma by inducing apoptosis and autophagy. In this case, intracellular calcium addition and degeneracy potential of mitochondrial membrane, ROS hyper generation and MAPK activation mediate the cytotoxic effects were studied and concluded with drug formulation studies. This study added a novel approach to the medicinal plant in cancer treatment.