Ichiro Amitani
Institution:
Kowalczykowski Lab
Davis, CA, United States
https://www.mysciencework.com/profile/ichiro.amitani
Summary
Published articles Show More
The BRC repeats of BRCA2 modulate the DNA-binding selectivity of RAD51.
Published in Cell
The breast cancer susceptibility protein, BRCA2, is essential for recombinational DNA repair. BRCA2 delivers RAD51 to double-stranded DNA (dsDNA) breaks through interaction with eight conserved, approximately 35 amino acid motifs, the BRC repeats. Here we show that the solitary BRC4 promotes assembly of RAD51 onto single-stranded DNA (ssDNA), but n...
Exploring protein-DNA interactions in 3D using in situ construction, manipulation and visualization of individual DNA du...
Published in Nature Protocols
In this protocol, we describe a procedure to generate DNA dumbbells -single molecules of DNA with a microscopic bead attached at each end-and techniques for manipulating individual DNA dumbbells. We also detail the design and fabrication of a microfluidic device (flow cell) used in conjunction with dual optical trapping to manipulate DNA dumbbells...
RecBCD enzyme switches lead motor subunits in response to chi recognition.
Published in Cell
RecBCD is a DNA helicase comprising two motor subunits, RecB and RecD. Recognition of the recombination hotspot, chi, causes RecBCD to pause and reduce translocation speed. To understand this control of translocation, we used single-molecule visualization to compare RecBCD to the RecBCD(K177Q) mutant with a defective RecD motor. RecBCD(K177Q) pause...
