Applicant
Life Technologies Corporation
Grantee
LIFE TECHNOLOGIES CORPORATION
Primary examiner
Maureen Wallenhorst
Application number
15891485
Kind
B2
Document number
10240193
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Sequencing Device

10240193 - 15891485 - USPTO

Application Feb 08, 2018 - Publication Mar 26, 2019

Jonathan Schultz Todd Roswech Jon A. Hoshizaki Albert L. Carrillo James A. Ball

Abstract

A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

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