PICHIA PASTORIS MUTANT STRAIN FOR EXPRESSING EXOGENOUS GENE
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Applicant
Ltd. Wilmar Biotechnology Research & Development Center Co.
Representative
Wilhelm Beier Dauster & Partner mbB Patentanwälte Ruff
Application number
EP19903304A1
Kind
A1
Document number
3910062
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PICHIA PASTORIS MUTANT STRAIN FOR EXPRESSING EXOGENOUS GENE

3910062 - EP19903304A1 - EPO

Application Dec 27, 2019 - Publication Nov 17, 2021

Wei WU Xiaojun DAI Haisheng CAO Meifeng ZHOU Qiwen NIU

Abstract

Provided is a Pichia pastoris mutant strain for expressing an exogenous gene. Specifically, provided is a Pichia pastoris mutant strain comprising, with respect to Pichia pastoris mutant strain GS115 or CICC32806, one or more of the following six mutations: BQ9382_C1-2260, EKK deletions at positions 308-310, a hypothetical protein; BQ9382_C1-3800, E129K, 60S ribosomal subunit assembly/exported protein LOCI; BQ9382 C1-5700, I312M, mitochondrial external NADH dehydrogenase, type II NAD(P)H:quinone oxidoreductase; BQ9382_C2-3950, Q145X, an essential protein having a binding partner Psrlp and used for completely activating a general stress response; BQ9382_C3-2220, E188K, a hypothetical protein; and BQ9382_C3-4370, W196X, orotidine 5\'-phosphate decarboxylase. The provided Pichia pastoris mutant strain is an effective commonly employed host for exogenous expression, and can efficiently express different proteins, especially phospholipase and lipase.

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