FLOW CYTOMETRY TESTING METHOD FOR LYMPHOCYTE IN IMMUNE CELL
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Applicant
Ltd. Mingdao Innovation Medical-Tech Co.
Representative
Lins & Partner Patent- und Rechtsanwälte PartGmbB Gramm
Application number
EP18941524A1
Kind
A1
Document number
3889576
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FLOW CYTOMETRY TESTING METHOD FOR LYMPHOCYTE IN IMMUNE CELL

3889576 - EP18941524A1 - EPO

Application Dec 01, 2018 - Publication Oct 06, 2021

Weili YAO

Abstract

The present application relates to the field of medicines, and in particular to a flow cytometric detection method for lymphocyte in immune cells. The method includes steps of: a) lymphocyte samples stained with immunofluorescent antibodies were added into the pre-cooled PBS-EDTA solution, and the cells were mixed and prepared for flow cytometry detection; b) starting up and warming up a flow cytometer system, adjusting the flow speed, then adding PBS-EDTA solution into a sample tube, and flushing a nozzle system of liquid stream; c) the sample of homogenous cells obtained in step a) was added to the sample tube and then tested. The present detection method can obviously separate cell subpopulations, so that the analysis and detection are more accurate, especially suitable for detecting cell subpopulations with small number of cells, and saving antibodies and reagents.

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Patent References

Patent Publication Date Title
CN201680006060 N/A N/A
CN201410486089 N/A N/A
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