& Diseased Brain, 75014 Paris, France

Protocol to study starvation-induced autophagy in developing rat neurons

Published in STAR Protocols

Autophagy is being involved in an increasing number of cellular pathways. It now appears that autophagy stimulation and inhibition have complex effects in neurons. Here, we present a simple yet powerful protocol to induce autophagy in primary neurons in culture by partial nutrient deprivation, in neurons with or without transfection of plasmids enc...