Department of Biochemistry, Faculty of Biological Sciences, Qu...

Fagonia olivieri Zygophyllaceae is used in Pakistan for management of diabetes and for treatment of stress related disorders of liver and kidneys The objective of present study was to evaluate antidiabetic activity of F olivieri using streptozotocin-nicotinamide induced diabetic rat model Powder of the whole plant of F olivieri was extracted with methanol FOME and the residue was fractionated with n-hexane FOHE chloroform FOCE ethyl acetate FOCE n-butanol FOBE and the remaining soluble aqueous fraction FOAE The extract fractions were evaluated for in vitro inhibition of -amylase and -glucosidase and for the antidiabetic studies in Sprague-Dawley rats FOAE was evaluated in 21 day chronic multiple dose study Glibenclamide 10 mg kg and FOAE were used at a concentration of 200 mg kg 164 8 g of catechin and 400 mg kg 329 6 g of catechin in streptozotocin STZ -induced diabetic rats Various serum parameters blood cell parameters and oxidative stress induced parameters of the liver and kidneys were investigated Results showed that aqueous fraction of F olivieri FOAE potentially inhibited activity of pancreatic -amylase intestinal maltase and sucrase Administration of FOAE 200 mg kg 400 mg kg to diabetic rats for 21 days increased body weight hemoglobin and its indices RBCs platelets lymphocytes and neutrophils against diabetic group In serum level of glucose transaminases ALP LDH total bilirubin creatinine and lipids decreased while insulin total protein and albumin increased in contrast to diabetic group Further level of cytokines TGF- 1 TNF- and IL-6 in serum and altered level of antioxidant enzymes glutathione and lipid peroxides of liver and kidney tissues restored towards control animals This study demonstrated that FOAE inhibited activity of digestive enzymes and exhibited antioxidant anti-inflammatory and antihyperglycemic activity in STZ-nicotinamide prompted diabetic rats and suggested that FOAE might be used as a therapeutic agent for the management of type-2 diabetes Copyright 2019 Elsevier B V All rights reserved

Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan. Electronic address: [email protected].

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Antioxidant, anti-inflammatory and hypoglycemic effects of Fagonia olivieri DC on STZ-nicotinamide induced diabetic rats...