Computation Institute, United States; Center for Research Informatics, United States.

A method for whole protein isolation from human cranial bone.

Published in Analytical Biochemistry

The presence of the dense hydroxyapatite matrix within human bone limits the applicability of conventional protocols for protein extraction. This has hindered the complete and accurate characterization of the human bone proteome thus far, leaving many bone-related disorders poorly understood. We sought to refine an existing method of protein extrac...