Clinic of Nephrology, University Hospital Aleksandrovska, Bulg...

Chronic kidney diseases CKDs are characterized by complex changes in cellular metabolism leading to oxidative stress OS i e increased production of oxidative radicals OR which can play a key role in a number of clinical complications of this pathology Trace elements selenium Se copper Cu and zinc Zn play a major role in the antioxidant defense system The antioxidant enzymes are superoxide dismutases SODs catalases CATs and glutathione peroxidases GSH-Pxs We evaluated serum hepcidin plasma selenium concentrations and the superoxide dismutase activity in 59 CKD patients on hemodialysis Patients were monitored at University Hospital Aleksandrovska Clinic of Dialysis Treatment and Clinic of Nephrology The results were compared with a matched by sex and age control group Serum hepcidin and SOD activity were determined by ELISA method Plasma selenium concentration was examined with the help of AAS Perkin-Elmer We determined CBC using hematology analyzer ADVIA 2120 Siemens Healthcare and biochemical parameters on automatic analyzer Dimension RxL MAX Siemens Healthcare of all participants The serum iron and TIBC levels were determined by AAS Perkin-Elmer For statistical analysis of the results we used Student s paired t-test and Pearson s correlation We found a statistically significant higher serum hepcidin levels in CKD patients on chronic dialysis 356 6 g L 52 5 g L compared to the control group 19 4 g L 2 7 g L P 0 001 Plasma selenium levels and SOD activity were decreased in CKD patients on on chronic dialysis 422 6 nmol L 51 1 nmol L 45 3 g mL 6 8 g mL compared to the control group 847 5 nmol L 75 4 nmol L 101 6 g mL 10 9 g mL P 0 001 The presence of anemic syndrome and oxidative stress are indisputable pathological conditions accompanying chronic kidney diseases A significant percentage of patients on dialysis are with abnormal levels of certain important trace elements - Zn Se Cu and magnesium and one recently discovered regulator of iron metabolism - hepcidin and between all of the following conditions are found interconnections and influences

Clinic of Nephrology, University Hospital Aleksandrovska, Bulgaria

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Hepcidin, Selenium and Superoxide Dismutase in Oxidative Stress and in Dialysis Patients