Bogomoletz Institute of Physiology, National Academy of Scienc...

The authors present the study of the level of expression of miR-196a in 74 patients with chronic viral hepatitis C with the 1st genotype of HCV based on previous experience in patients with antiviral therapy regimens containing interferon The patients were divided into two groups depending on the previous experience of antiviral therapy with circuits containing interferon 21 patients who failed after antiviral therapy regimens containing interferon group 1 and the comparison group group 2 53 naive patients To study the level of expression of miR-196a miR-196a a two-stage study according to the manufacturer s protocol was used First total RNA was isolated from the plasma by the method of phenol-chloroform extraction Futher reverse transcription was performed using a kit for reverse transcription of miR TaqMan Applied Biosystems USA specific loop primers to achieve mature miRNA snRNA U6 as an endogenous control gene and 10 ng of total RNA Real-time quantitative PCR was performed using TaqMan miRNA analysis In order to optimize the prediction of response to antiviral therapy and the use of optimal treatment regimens for problem patients with treatment failure regimens containing interferon analysis using ROC curves was used The average level of expression of miR-196a in patients with chronic hepatitis C was evaluated In the 1st group of patients it was 0 011 IQR 0 002 0 310 and in the comparison group 0 346 IQR 0 054 1 239 at p 0 012 by U criterion The conducted ROC analysis showed that the studied miR-196a could differentiate patients with chronic viral hepatitis C with HCV genotype 1 depending on previous experience of antiviral therapy namely patients with treatment failure regimens containing interferons and naive AUC 0 688 95 CI 0 570-0 791 p 0 017 J 0 40 Se 57 14 Sp 83 02 It gives additional opportunities for correction of therapeutic tactics Therefore the level of expression of miR-196a -1 75 may be an additional biomarker in the pathogenesis of HCV-infection which can then be used in the monitoring and treatment of patients Low expression of miR-196a may be the basis for prescribing more effective direct-acting antiviral therapy to patients and will allow personalizing therapeutic tactics in patients with chronic viral hepatitis C

Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

About this institution

Publications Show all

The level of expression of miR-196a in patients with chronic viral hepatitis C with the first genotype of HCV according ...